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Showcase Health Sciences

The Sea as a Source of Novel Antiparasitic Compounds

The Sea as a Source of Novel Antiparasitic Compounds

Primary Author: Rachel Relat

Faculty Sponsor: Roberta O’Connor

 

Primary College/Unit: College of Veterinary Medicine

Category: Medical and Life Sciences

Campus: Pullman

 

Abstract:

 

PRINCIPLE TOPIC

Cryptosporidium, an intracellular parasite that causes severe diarrhea, is now recognized as a leading cause of waterborne disease worldwide, infecting both humans and valuable livestock.  Cryptosporidium infection is particularly devastating in children as it causes life-threatening diarrhea, along with developmental delays including growth stunting and cognitive impairment. Cryptosporidium is an especially important disease of immunocompromised individuals of all ages, leading to chronic, potentially fatal diarrhea. No effective treatment exists for many human or veterinary patients diagnosed with Cryptosporidium.

METHODS

To address this medical imperative, we designed a high throughput screen to test thousands of unique compounds, with the goal of discovering new, potent, anti-Cryptosporidium drugs and drug scaffolds. Our collaborators at the Harbor Branch Oceanographic Institute (HBOI) have amassed a library of over 125 natural compounds, and 6500 highly enriched fractions from extracts of marine organisms. We began screening this library as it contains compounds and fractions produced by sessile oceanic animals which frequently use chemical defenses.  We screened greater than 3,600 partially purified extracts from the HBOI library by infecting cells with Cryptosporidium parvum, allowing replication, and then treating with a specific highly enriched fractions or compounds, and then measuring parasite growth after 48 hours.

RESULTS

We identified 10 extracts derived from sessile marine organisms that effectively inhibit (>80% inhibition) Cryptosporidium without damaging the host cells in which these parasites live. This work will significantly contribute to the goal of discovering effective treatments against Cryptosporidium specifically, and further the search for novel anti-parasitic drugs.

 

‘We’re Here to Help’: Students of Color Experiences and Satisfaction with Health and Emergency Services at Washington State University

‘We’re Here to Help’: Students of Color Experiences and Satisfaction with Health and Emergency Services at Washington State University

Primary author: Brianne Posey
Faculty sponsor: Patricia Maarhuis

Primary college/unit: Cougar Health Services/Health Promotion
Category: Physical and Social Sciences

Campus: Pullman

Abstract:

Background: People of color may face barriers to care services. Some of these barriers stem from financial and geographic disadvantage, while other barriers stem from negative experiences with practitioners and staff. Patient satisfaction with care services is an important factor in retention. Previous studies have concluded that patient experiences and satisfaction scores significantly predict whether a patient maintains a continuing relationship with a practitioner. Research Objectives: Researchers sought to examine the self-reported experiences and satisfaction scores of minority students at Washington State University. Data: A survey questionnaire was distributed to 200 students of color regarding their experiences and satisfaction with four Washington State University, Pullman campus and community emergency and health care services: 1) Cougar Health Services Medical Clinic (CHS), 2) Counseling and Psychological Services (CAPS), 3) Washington State University Police Department (WSU PD), 4) Pullman Police Department (Pullman PD). Analysis: Quantitative descriptive analysis was performed along with qualitative thematic analysis. Results: Participant experiences such as friendly staff, trauma informed interviewing, and culturally respectful and inclusive language were associated with higher satisfaction scores and students being more likely to return. Oppositely, experiences such as long wait times, incorrect diagnosis, and high cost of treatment were associated with lower satisfaction scores and students being less likely to return. Conclusion: This study provides data about factors important to patient satisfaction with care services for a selected group of college students. Recommendations for improving satisfaction among students of color are provided.

A Feasibility Study On 3D Printed Biopsy Needles: Pronged Tips And INternal Features Affect On Tissue Yield

A Feasibility Study On 3D Printed Biopsy Needles: Pronged Tips And INternal Features Affect On Tissue Yield

Primary Author: Anika VanDeen

Faculty Sponsor: Roland Chen

 

Primary College/Unit: Voiland College of Engineering and Architecture

Category: Engineering and Environmental Science

Campus: Pullman

 

Abstract:

 

Principal Topic

Cancer is an ever-prevalent disease accounting for a large portion of medical services. With an aging world population, it is imperative to have efficient and non-invasive diagnostics. Biopsy methods such as core needle biopsy (CNB) and fine needle aspiration (FNA) are some of the most common practices used to garner an accurate diagnosis.

Methods

This study explores the feasibility of using 3D printing to fabricate biopsy needles with advanced designs that cannot be manufactured using traditional methods. We focus on enhancing the needle tip geometry as well as the addition of an internal feature to help improve tissue yield amounts. Four needle designs were tested, two with a bevel shaped tip, and two with a five-pronged tip, both with and without the internal feature.

 

Results/Implications

Average total yield for the beveled needle with no feature (n=10), was 3.217 mg (s=0.9104), and for the beveled needle with feature, five-pronged needle without feature, and the five-pronged needle with feature (n=22), measured 8.31 mg (s=3.07 mg), 16.16 mg (s=12.21), and 26.34 mg (s=12.94), respectively. There is a statistical difference in yield tissue amounts between the five-prong needle with and without feature (p=0.01). The pronged-tip design with the internal feature is shown to have a significant effect on tissue sampling efficiency. The 3D printed needles do create higher insertion force than that of the stainless-steel needles. This study demonstrates the feasibility of using 3D printing to fabricate biopsy needles with enhanced tissue sampling efficiency.

 

Mental Health First Aid: Increasing student pharmacist preparedness to respond through simulation

Mental Health First Aid: Increasing student pharmacist preparedness to respond through simulation

Primary Author: Easton Stoner

Faculty Sponsor: Jennifer Robinson

 

Primary College/Unit: College of Pharmacy and Pharmaceutical Sciences

Category: Medical and Life Sciences

Campus: Pullman

 

Abstract:

 

Suicidal ideation, depression, and anxiety are common mental health conditions that are present in our communities and classrooms.  Student pharmacists have historically reported feeling under prepared to support those in need during a mental health crisis. Research teams from Washington State University College of Pharmacy and Pharmaceutical Sciences (CPPS) collaborated with The University of Sydney School of Pharmacy to establish a meaningful educational intervention focused on training student pharmacists with the skills and confidence needed to respond to a mental health crisis.

In the of fall 2019, a Mental Health Human Patient Simulation activity was integrated into an already established Mental Health First Aid elective course with an enrollment of 60 doctor of pharmacy students. For the simulation, students were placed into groups of 5-6 and each group participated in three patient simulations: depression with suicidal ideation, depression with no suicidal ideation, and a manic episode. To measure the effectiveness of the course overall, students completed a pre-course and post-course survey to rate confidence in providing mental health first aid.

Results show that students leave the course feeling more confident and rated their preparedness to provide mental health first aid much higher post- course when compared to pre-course ratings. With the rates of suicidal ideation and deaths by suicide being so high, the implications and applicability of this research can be applied to students in all fields in the hope of decreasing the incidence of death by suicide in our communities.

 

Modeling the Impact of Staff to Patient Ratios on MRSA Acquisition in an Intensive Care Unit

Modeling the Impact of Staff to Patient Ratios on MRSA Acquisition in an Intensive Care Unit

Primary Author: Stephanie Sikavitsas Johnson

Faculty Sponsor: Eric Lofgren

 

Primary College/Unit: College of Veterinary Medicine

Category: Medical and Life Sciences

Campus: Pullman

 

Abstract:

 

Staff-to-Patient ratios in hospitals impact several hospital acquired infections (HAIs). Intuitively, increasing the number of nurses and doctors in an intensive care unit (ICU) should impact rates of HAI transmission by reducing the number of patients per provider, allowing less patient-patient interaction. Designing a study to determine how different staffing ratios would affect HAIs is difficult to in person because of lots of different factors that make each hospital and unit unique. Mathematical modeling can be used instead to simulate how staffing ratios affect methicillin-resistant S. aureus (MRSA) being acquired in a 15-bed ICU.

 

We varied the number of doctors in the ICU from one to three. For each doctor scenario, the nurse -patient ratio was 1:1, 1:2.5, 1:3. And 1:5. We ran each model 1000 times, with each time simulating 1 year in the ICU. The outcome was the mean number of MRSA acquisitions in one year for each of the 12 models. Our baseline model we compared everything to was the single doctor, 1:3 nurse-patient ratio model.

 

Adding more doctors slightly decreased the number of acquisitions, with the steeper decline going from one doctor to two. Going from a 1:3 nursing ratio to 1:1 ratio decreased MRSA acquisition by 64%. Increasing to a 1:5 ratio raised acquisitions 21.5%. These results suggest reduction in provider contact may have an impact on HAI rates. Appropriate staffing should be considered in infection control guidelines, and the cost of staffing be weighed against its impact on infection prevention.

 

Optimizing the Production of Stem-cell-based Cartilage for Arthritis Treatment

Optimizing the Production of Stem-cell-based Cartilage for Arthritis Treatment

Primary Author: Olivia Reynolds

Faculty Sponsor: Bernard Van Wie

 

Primary College/Unit: Voiland College of Engineering and Architecture

Category: Engineering and Environmental Science

Campus: Pullman

 

Abstract:

 

Principle topic

Osteoarthritis (OA), the degradation of articular cartilage tissue which lines joints, affects 30 million adults in the United States. There are few effective treatment options for OA; however, stem-cell-based therapy is a promising emerging treatment as stem cells can form new healthy cartilage. The use of chemical growth factors and anti-inflammatory compounds, as well as the co-culture of stem cells with native cartilage cells can enhance cartilage formation and promote the production of critical structural proteins such as glycosaminoglycans (GAG) and collagen. However, the optimum combination of these factors is unknown. Thus, this work investigated the combined roles of the growth factor dexamethasone, the anti-inflammatory compound alpha-tocopherol, and the ratio of stem cells to cartilage cells in an effort to determine an optimum combination and improve the quality of engineered cartilage tissue.

 

Method

In this work, dexamethasone concentration, alpha-tocopherol concentration, and stem cell percentage were varied using a Box-Behnken fractional factorial design scheme. Following 21 days of cell culture, total collagen, GAG, and DNA were measured for each experimental group to quantify the amount of cartilage-specific proteins produced. Data were analyzed to determine the culture conditions which resulted in the maximum quantity of collagen and GAG as these proteins indicate cartilage formation.

 

Results/implications

The results of this study showed that the highest protein production occurred at low stem cell percentages, low to moderate anti-inflammatory concentrations, and moderate growth factor concentration. These results may be used to improve the production of cartilage for applications in OA treatment.

 

Effects of Garlic Extract Released from Calcium Phosphate Scaffolds for Bone Tissue Engineering Applications

Effects of Garlic Extract Released from Calcium Phosphate Scaffolds for Bone Tissue Engineering Applications

Primary Author: Ashley Vu

Faculty Sponsor: Susmita Bose

 

Primary College/Unit: Voiland College of Engineering and Architecture

Category: Medical and Life Sciences

Campus: Pullman

 

Abstract:

 

PRINCIPAL TOPIC

Bone is a constantly remodeling tissue comprised of osteoblast cells which form bone and osteoclast cells which remove old bone through resorption. Garlic is historically known for the prevention and treatment of diseases however knowledge is limited regarding bone health. Animal studies have shown garlic minimizes bone loss through increasing estrogen levels and reducing osteoclast bone resorption. Excessive bone loss can cause porous, brittle bones, commonly known as osteoporosis, which lead to high fracture risks. Utilizing natural alternatives to synthetic medicines can reduce physiological rejection while maintaining relief to ailments and diseases.

 

METHOD

One of the most well-known sulfur compounds extracted from garlic is allicin. The objective is to understand the effects of allicin release on the bone remodeling process. The hypothesis is allicin will show no cytotoxic effects to osteoblast cells and reduce osteoclast resorption. Allicin was extracted from pure garlic powder and loaded onto calcium phosphate scaffolds, mimicking bone tissue composition. Cellular and scaffold surface morphology were imaged post cell culture as well histological staining of an in vivo rat distal femur model.

 

RESULTS/IMPLICATIONS

Results show allicin has no cytotoxic effects on osteoblast morphology and a reduction of osteoclast resorption pit formation. Allicin also shows significantly enhanced collagen formation in vivo, indicating another avenue for improved bone healing. With these results, further knowledge is gained on the ability for garlic to improve bone health in bone tissue engineering applications.