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Showcase Marilyn Reed

Dissecting the genetic architecture of Aphanomyces root rot resistance in lentil by QTL mapping and genome-wide association study

Dissecting the genetic architecture of Aphanomyces root rot resistance in lentil by QTL mapping and genome-wide association study

Primary author: Yu Ma
Co-author(s): Afef Marzougui; Clarice Coyne; Sindhuja Sankaran; Dorrie Main; Lyndon Porter; Deus Mugabe; Jamin Smitchger
Faculty sponsor: Dorrie Main

Primary college/unit: Agricultural, Human and Natural Resource Sciences
Campus: Pullman

Abstract:

Lentil (Lens culinaris Medikus) is an important source of protein for people in developing countries. Aphanomyces root rot (ARR) has emerged as one of most devastating diseases affecting lentil production. In this study, we applied two complementary QTL analysis approaches to unravel the genetic architecture underlying this complex trait. A recombinant inbred line (RIL) population and an association mapping population were genotyped using genotyping by sequencing (GBS) to discover novel SNPs. QTL mapping identified 19 QTL associated with ARR resistance, while association mapping detected 38 QTL and highlighted accumulation of favorable haplotypes in most of the resistant accessions. Seven QTL clusters were discovered on six chromosomes and five putative genes involved in plant disease response were detected. Expression analysis revealed four of them, encoding an ABC transporter A family protein, a cytochrome P450 family 71 protein, a chalcone-flavanone isomerase family protein, and pectin esterase, were differentially expressed between resistant and susceptible accessions. This indicates genes involved in secondary metabolism and cell wall modification are potentially associated with ARR. Our findings provide valuable insight into the genetic control of ARR and genetic and genomic resources developed here can be used to accelerate development of lentil cultivars with high levels of partial resistance to ARR.

Hierarchically Porous M-N-C (M = Co and Fe) Single-Atom Electrocatalysts for Fuel Cells

Hierarchically Porous M-N-C (M = Co and Fe) Single-Atom Electrocatalysts for Fuel Cells

Primary author: Zhaoyuan Lyu
Faculty sponsor: Yuehe Lin

Primary college/unit: Voiland College of Engineering and Architecture
Campus: Pullman

Abstract:

The integration of hydrogen into power generation applications is critical for an environmentally friendly and sustainable energy pattern. Fuel cells are considered as one of the cleanest energy conversion technologies. The efficiency and cost of fuel cells, however, are still hurdled by the development of cost-effective catalysts that reduces oxygen at the cathode side. Currently, there is an intensive research effort for highly efficient electrocatalysts based on low-cost and earth-abundant elements. Improving non-precious metal catalysts require rational control over their size, shape, composition, and structure. In particular, single-atom catalysts (SACs) show great promise owing to their high catalytic activity, stability, selectivity, and 100?% atom utilization. Through innovative synthesis methods, we have developed a universal strategy to design and construct hierarchically porous SACs with highly active sites, MN2 (M = Fe or Co), which outperform the commercial precious-metal catalysts and show great potential for practical proton-exchange membrane fuel cells. Combining advanced characterization techniques and theoretical simulation, we further unravel the origin of the high catalytic activity of SACs at the atomic level. The findings shed light on the catalytic reaction mechanism of SACs and may help future development of low-cost and highly efficient fuel cell catalysts.

Breastfeeding patterns in four cultures: A cross cultural analysis

Breastfeeding patterns in four cultures: A cross cultural analysis

Primary author: Courtney Love
Co-authors: Katherine Flores, Beatrice Caffe, Avery Lane, Courtney Helfrecht, Courtney L. Meehan
Faculty sponsor: Courtney Meehan

Primary college/unit: Arts and Sciences
Campus: Pullman

Abstract:

Breastfeeding, widely acknowledged to have significant benefits to infant growth and development, maternal-infant bonding, and infant immunity, is also one of the most intensive and energetically expensive forms of maternal investment. Despite these noteworthy benefits, we lack foundational data regarding the underlying daily structure (e.g., frequency and number of bouts, temporal and developmental variation) of breastfeeding, particularly in cross-cultural perspective. Here we present data on breastfeeding structure in four cultures and test the broad hypothesis that maternal and infant life history characteristics and allomaternal investment are related to infant nursing patterns. Data were analyzed on 226 infants and children via naturalistic focal follow behavioral observations, which recorded infant behavior every 30 seconds across daylight hours, among hunter-gatherers and horticulturalists in the Central African Republic, agro-pastoralists in Ethiopia, and Washington/Idaho women living in a semi-rural community in the US. Our results support the hypotheses that several life history characteristics (e.g., infant age, sex, and allomaternal network size) are associated with nursing frequency. Understanding the daily structure of breastfeeding across subsistence patterns can help us understand cross-cultural variation, in addition to illustrating how life history traits influence maternal investment strategies on a critical investment pattern with clear fitness consequences.

PRKAR1A deficiency delays postnatal heart growth

PRKAR1A deficiency delays postnatal heart growth

Primary author: Yuening Liu
Faculty sponsor: Zhaokang Cheng

Primary college/unit: College of Pharmacy and Pharmaceutical Sciences
Campus: Spokane

Abstract:

Aims: Protein kinase A (PKA) activity is pivotal for functioning of the human heart, and its dysregulation has been implicated in cardiac pathologies. PKA regulatory subunit 1a (R1a, encoded by the PRKAR1A gene) is highly expressed in the heart, and controls PKA kinase activity. Patients with PRKAR1A mutations are often diagnosed with Carney complex (CNC) in young adults, and may die prematurely from cardiac complications such as heart failure. However, no cardiac defect has been reported in adult animal models of PRKAR1A deficiency.
Methods and Results: To investigate the impact of PRKAR1A deficiency, we generated cardiac-specific PRKAR1A heterozygous knockout mice by breeding the floxed PRKAR1A mice with the Mlc2v-Cre mice. We also studied a cohort of young CNC patients with PRKAR1A mutations or deletions. Ablation of the PRKAR1A gene in mice increased cardiac PKA activity, reduced heart weight and cardiomyocyte size without altering contractile function at 3 months of age. Importantly, left ventricular mass was reduced in young patients diagnosed with CNC. Cardiomyocyte hypertrophy in response to activation of the a1-adrenergic receptor, which is necessary for heart growth after birth, was completely abolished by silencing of PRKAR1A, or stimulation with the PKA activator forskolin. Mechanistically, depletion of PRKAR1A provoked PKA-dependent phosphorylation of the mitochondrial fission protein Drp1 at S637, leading to suppression of mitochondrial fission and inhibition of cardiomyocyte hypertrophy.

Conclusions: PRKAR1A deficiency impedes postnatal myocardial development and physiological hypertrophy through modulation of mitochondrial dynamics. These findings provide a potential novel mechanism for the cardiac manifestations associated with CNC.

Sales-Service Ambidexterity on Salesperson Performance: Do Role Characteristics Play a Role?

Sales-Service Ambidexterity on Salesperson Performance: Do Role Characteristics Play a Role?

Primary author: Muzi Liu
Co-author(s): Muzi Liu; Pavan Munaganti
Faculty sponsor: Dr. Babu John Mariadoss

Primary college/unit: Carson College of Business
Campus: Pullman

Abstract:

Firms are increasingly requiring their frontline employees to play an ambidextrous role, that is, engage in “both sales and service activities regardless of formal title or position” (Rapp et al. 2017, 59). While ambidexterity refers to the simultaneous pursuit of dual, and often conflicting strategic goals (Simsek 2009), service researchers have focused on service-sales ambidexterity by examining service personnel who perform sales activities (e.g., Gwinner et al. 2005; Jasmand, Blazevic, & de Ruyter 2012), and sales researchers have focused on sales-service ambidexterity by examining service performance within the salesforce (e.g., Ahearne, Jelinek, & Jones 2007). Recent research (e.g., Rapp et al. 2017) suggests that the capacity of employees to function ambidextrously depends on whether their dual sales and service roles are perceived as a stressor, and whether firms can create conditions facilitating role integration and reconciling competing individual-level role demands, leading to successful alignment between customer service and sales. Extant literature (e.g., Singh 1998; Johnson, Anderson, & Fornell 1995) suggests that the blurring of roles between sales and service personnel can have implications on employee role characteristics such as role conflict, role ambiguity and role overload, and ultimately affect performance. The purpose of this research is to examine the effect of ambidexterity on performance, through the mediating effect of salesperson role characteristics. In two studies, we find that sales-service ambidexterity leads to increased perceived role conflict, ambiguity, and overload amongst frontline employees, and ultimately in diminished frontline employee performance.

Novel Deep Eutectic Solvent for Native Lignin Extraction Through Heterocycle Induced Interaction

Novel Deep Eutectic Solvent for Native Lignin Extraction Through Heterocycle Induced Interaction

Primary author: Kuan-Ting Lin
Faculty sponsor: Dr. Xiao Zhang

Primary college/unit: Voiland College of Engineering and Architecture
Campus: Tri-Cities

Abstract:

Plant biomass, constructed by lignocellulose, is the largest renewable source for biofuel and bioproducts to replace petroleum products. To facilitate the process of lignocellulose to product, fractionation of lignocellulose is performed as the primary step to improve carbohydrate utilization, and isolate lignin for valorization. Current industrial pretreatment and/or pulping approach apply harsh processing conditions to extract lignin from the biomass matrix. In these harsh conditions, lignin-carbohydrate complexes are not completely broken, resulting in a significant amount of residual saccharides and process chemicals. Deep eutectic solvent (DES) is a promising alternative method to effectively extract lignin from lignocellulosic biomass. Previous studies have shown DES generally yielded good lignin purity and yield. Although DES extracted lignins are relatively pure, the process is known for acid hydrolysis of the ether linkages in lignin which caused a large degree of structural changed. In this study, we hypothesized that design a DES with heterocycles enable a selective extraction of lignin from plant biomass, meanwhile the function of HBD under different reaction condition control the cleavage of ether linkages that produce lignin with tailor structural properties (ether linkage and depolymerization).

Student Use of the Washington State Twin Registry: An Analysis of Low Back Pain and Body Mass Index in Monozygotic and Dizygotic Twins

Student Use of the Washington State Twin Registry: An Analysis of Low Back Pain and Body Mass Index in Monozygotic and Dizygotic Twins

Primary author: Andrew Liechty
Faculty sponsor: Glen Duncan

Primary college/unit: Elson S. Floyd College of Medicine
Campus: Everett

Abstract:

In cohort studies, it can often be difficult to separate the question of nature vs nurture in the development of a particular disease. For this, Monozygotic twins (i.e. identical twins) allow us a convenient model to analyze various outcomes of interest between two individuals as they control for the genetic aspects. Students at WSU have access to the Washington State Twin Registry which contains a database of Monozygotic and Dizygotic Twin pairs that have answered questionnaires about health, education, and income for many years. I analyzed the relationship between low back pain and body mass index. I hypothesized that an increase in body mass index would lead to an increase in low back pain. We found that there was not a step wise increase in BMI and LBP. In fact, in monozygotic twins who were discordant for BMI (one normal weight and one obese) there was no statistical difference in LBP. We did find however, that there may be a genetic link between the two. We also found that Moderate to Vigorous Physical Activity is associated with LBP, however these may also be linked through a common genetic element. The Washington State Twin Registry is an untapped resource for undergraduate, graduate, and professional students as it allows us to account for genetics while analyzing a variety of factors.

BAM!: Chicago’s Black Arts Movement

BAM!: Chicago’s Black Arts Movement

Primary author: Thabiti Lewis
Co-author(s): Pavithra Narayanan

Primary college/unit: Arts and Sciences
Campus: Vancouver

Abstract:

The film examines the Black Arts Movement in Chicago, which is an epicenter of the black diaspora. The film “BAM! Chicago’s Black Arts Movement” features interviews with Museum director Carol Adams, publisher and poet Haki Madhubuti, Safisha Madhubuti, Eugene Redmond, Mwata Bowden, Angela Jackson and many other artists and scholars. The film introduces viewers to the history of Chicago’s Black Arts Movement (BAM) and reflects on the extensive national and international impact of Chicago’s Black writers, musicians and community organizers and the organizations and institutions that they supported and founded including the Association for the Advancement of Creative Musicians (AACM), the Organization of Black American Culture (OBAC), Negro Digest/Black World, Ebony Talent Agency (ETA), the DuSable Museum, Third World Press, Johnson Publishing, Kuumba Theatre, and the South Side Community Arts Center.
The film explores the 1960s era of art and politics and why Chicago emerged as one of the most important cities and was able to be such an influential matrix for Black communities across the country seeking to duplicate Chicago’s institutional building and arts scene.
Preview of Film: https://vimeo.com/295695342
Runtime: 55 minutes

REMOTE: VOICES OF THE WILDERNESS

REMOTE: VOICES OF THE WILDERNESS

Primary author: DJ Lee

Primary college/unit: Arts and Sciences
Campus: Pullman

Abstract:

The Selway-Bitterroot Wilderness, one of the most remote landscapes in the U.S., stretches across Idaho and Montana in the Northern Rockies, where it borders the Frank Church River of No Return and the Gospel Hump Wildernesses to form the largest roadless wildland in the lower 48 states at over 5 million acres. I, my co-PI at the University of Idaho, WSU graduate students, and digital librarians, received the prestigious NEH Collaborative Research Grant for the Selway-Bitterroot Wilderness History Project in 2010. We collected 16 linear feet of photographs, maps, and documents and conducted 50 oral history interviews—the first ever digital and analog archive of a single Wilderness area. In 2014, our project was honored during the 50th anniversary of the Wilderness Act, solidifying its importance to Wilderness studies in the U.S. and worldwide. Since then, the value of Wilderness has increased globally as it becomes clear how vital large, roadless wildlands are for carbon sequestration, wild animal habitat, and biodiversity, not to mention spiritual renewal. Two creative products designed to reach larger audiences have grown from the project: 1) a podcast of curated stories from oral history interviewees with photo slideshows; 2) my creative nonfiction book, REMOTE: FINDING HOME IN THE BITTERROOTS from Oregon State University Press, forthcoming March 3, 2020. REMOTE was featured as one the most anticipated books by the Pacific Northwest Bookseller’s Association in October 2019. My Showcase Poster will focus on the creative products and processes of the Selway-Bitterroot Wilderness Project.

Acute Effects of High-Potency Cannabis on Everyday Life Cognition

Acute Effects of High-Potency Cannabis on Everyday Life Cognition

Primary author: Emily LaFrance
Faculty sponsor: Carrie Cuttler, PhD

Primary college/unit: Arts and Sciences
Campus: Pullman

Abstract:

Previous research indicates that tetrahydrocannabinol (THC), the primary constituent in cannabis, impairs memory and may affect risky decision-making. A small body of research further suggests that cannabidiol (CBD), which is also found in cannabis, may offset memory impairments. However, research demonstrating these effects has primarily utilized low THC ( 20% THC) cannabis and cannabis concentrates (> 60% THC) on cognition, and ii) to assess the role of CBD in the acute effects of cannabis on cognition. To achieve these aims, a between-subjects field experiment using Zoom videoconferencing software was employed. Participants in this study purchased a specific type of cannabis and then engaged in videoconferencing testing session from their home via Zoom. During this session, participants were observed while inhaling cannabis or remaining sober, and then completed tests of their memory (prospective, source, temporal order, and false memory) and decision making (risky choice framing, consistency in risk perception, resistance to sunk cost, and over/under confidence). Most of these tests measure aspects of cognition that have never been investigated under conditions of acute cannabis intoxication. Preliminary results indicate that cannabis intoxication impairs source memory and false memory but does not significantly impact decision making. CBD does not appear to offset these impairments. Results of this study have implications for cannabis users, and health practitioners working with cannabis-using populations.