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Optimizing the Production of Stem-cell-based Cartilage for Arthritis Treatment

Optimizing the Production of Stem-cell-based Cartilage for Arthritis Treatment

Primary Author: Olivia Reynolds

Faculty Sponsor: Bernard Van Wie

 

Primary College/Unit: Voiland College of Engineering and Architecture

Category: Engineering and Environmental Science

Campus: Pullman

 

Abstract:

 

Principle topic

Osteoarthritis (OA), the degradation of articular cartilage tissue which lines joints, affects 30 million adults in the United States. There are few effective treatment options for OA; however, stem-cell-based therapy is a promising emerging treatment as stem cells can form new healthy cartilage. The use of chemical growth factors and anti-inflammatory compounds, as well as the co-culture of stem cells with native cartilage cells can enhance cartilage formation and promote the production of critical structural proteins such as glycosaminoglycans (GAG) and collagen. However, the optimum combination of these factors is unknown. Thus, this work investigated the combined roles of the growth factor dexamethasone, the anti-inflammatory compound alpha-tocopherol, and the ratio of stem cells to cartilage cells in an effort to determine an optimum combination and improve the quality of engineered cartilage tissue.

 

Method

In this work, dexamethasone concentration, alpha-tocopherol concentration, and stem cell percentage were varied using a Box-Behnken fractional factorial design scheme. Following 21 days of cell culture, total collagen, GAG, and DNA were measured for each experimental group to quantify the amount of cartilage-specific proteins produced. Data were analyzed to determine the culture conditions which resulted in the maximum quantity of collagen and GAG as these proteins indicate cartilage formation.

 

Results/implications

The results of this study showed that the highest protein production occurred at low stem cell percentages, low to moderate anti-inflammatory concentrations, and moderate growth factor concentration. These results may be used to improve the production of cartilage for applications in OA treatment.

 

Exploring the Effectiveness of Academic Probation/Reinstatement Interventions on Academic Success Behaviors and Strategies of Undergraduate Students

Exploring the Effectiveness of Academic Probation/Reinstatement Interventions on Academic Success Behaviors and Strategies of Undergraduate Students

Primary Author: Katie Reilly

Faculty Sponsor: Olusola Adesope

 

Primary College/Unit: College of Education

Category: Arts and Education Sciences

Campus: Pullman

 

Abstract:

PRINCIPAL TOPIC

Undergraduate students who are on academic probation/reinstatement due to falling short of expected GPA standards set by colleges and universities are at high risk of dropping out or being dismissed. To retain these students and to support students at-risk of academic failure, many colleges and universities have focused on providing interventions to increase retention and academic success. The goal of this pilot study was to examine the impact of academic support interventions that possibly contribute to the academic success and retention of students who are not meeting WSU academic standards.

 

METHOD

This study sought to answer the following research questions: 1) What are the overall impacts of academic probation interventions on the academic success strategies and behaviors of students on academic probation? and 2) Which intervention(s) have the most significant impact on the academic success strategies and behaviors of students on academic probation? Using an experimental within-subjects design, this study investigated the impact of three support interventions: an academic success course, academic coaching, and workshops.

 

RESULTS AND IMPLICATIONS

Initial results showed increases in positive academic success behaviors and strategies for all three interventions, however academic coaching interventions saw higher gains in academic success strategies and behaviors than students enrolled in an academic success course or students assigned to workshops. While research has mostly focused on impacts academic success courses have on supporting students on academic probation/reinstatement, this study suggests that research should begin exploring additional methods of support that may ultimately be more constructive to this special population.

 

Exploring and Quantifying Unsystematic Risk in US Hotel REITs

Exploring and Quantifying Unsystematic Risk in US Hotel REITs

Primary Author: Hongwei Zhao

Faculty Sponsor: Ming-Hsiang Chen

 

Primary College/Unit: Carson College of Business

Category: Business, Communication, and Politial Sciences

Campus: Pullman

 

Abstract:

 

Principal topic:

Although Real Estate Investment Trusts (REITs) were originally conceptualized as mechanisms for individual investors to participate in institutional real estate, the ownership of REIT stocks has recently become dominated by institutional investors. Meanwhile, the hybrid nature of REITs is problematic for investors, particularly less diversified, individual investors who cannot mitigate unsystematic risk. This study included three sections intended to deconstruct unsystematic risk and enable investors to make more informed decisions in their investment portfolios.

Method:

Section one investigated unsystematic risk by selecting all publicly-traded company daily returns from January 2001 to December 2018 and running Fama-French model. Section two conducted a parallel analysis of unsystematic risk and institutional ownership. Section three investigated herding behavior in the Hotel REIT sector by parallel regression.

Results/implications:

Hotel REIT stocks have experienced wide variations in unsystematic risk between 14% and 92.5%. The trend toward institutional ownership of stocks is shown to have a significant negative impact on the levels of unsystematic risk. The implications remain to be identified as to whether higher levels of institutional ownership improve management efficiency and account for more monitoring power, in which price reflects more information and contains less individual specific risk. The unsystematic risk from REIT market returns is significantly lower than the one from the entire market returns, supporting that herding behavior in the Hotel REIT sector might “systematize” a portion of the unsystematic risk. Tracking investor sentiment may be one of factors explaining unsystematic risk for Hotel REITs.

 

Comparison of Microwave and Conventional Thermal Pasteurization of Frozen Green Beans

Comparison of Microwave and Conventional Thermal Pasteurization of Frozen Green Beans

Primary Author: ZHI QU

Faculty Sponsor: Juming Tang

 

Primary College/Unit: Agricultural, Human and Natural Resource Sciences

Category: Agricultural and Natural Resource Sciences

Campus: Pullman

 

Abstract:

 

PRINCIPAL TOPIC

Pasteurization is the process to eliminate pathogens and extend shelf life of food products. Consumer’s desire for high quality food and convenience has been a major driver for advancements of processing technologies. Microwave pasteurization can provide more rapid heating and better heating uniformity compared to conventional thermal pasteurization, thus holds potential to produce better quality vegetable products, such as fresh appearance and texture. The objective of this research was to study the influence of microwave and conventional thermal pasteurization on quality of green beans during storage at various cold chain temperatures.

METHOD

Thawed frozen green beans were vacuum sealed in 8 oz polymer trays, pasteurized in a pilot-scale 915 MHz Microwave Assisted Pasteurization System (MAPS) and conventional water bath (WB). The processed samples were stored at 10 and 2 °C. Color and chlorophyll content of green beans were quantified to elucidate the quality of the food.

RESULTS/IMPLICATIONS

For green beans, chlorophyll and green color suffered greater degradation when pasteurized using WB. During storage, under both temperatures, microwave pasteurized samples showed better color retention and higher chlorophyll content. And, microwave pasteurized green beans can be preserved for a longer time at 2°C (100 days) than WB heated one (80 days) with no package swelling observed. This implicates that microwave pasteurization might be a potential alternative to produce safe, high-quality vegetable products and preserve the quality during storage.

 

Effects of Garlic Extract Released from Calcium Phosphate Scaffolds for Bone Tissue Engineering Applications

Effects of Garlic Extract Released from Calcium Phosphate Scaffolds for Bone Tissue Engineering Applications

Primary Author: Ashley Vu

Faculty Sponsor: Susmita Bose

 

Primary College/Unit: Voiland College of Engineering and Architecture

Category: Medical and Life Sciences

Campus: Pullman

 

Abstract:

 

PRINCIPAL TOPIC

Bone is a constantly remodeling tissue comprised of osteoblast cells which form bone and osteoclast cells which remove old bone through resorption. Garlic is historically known for the prevention and treatment of diseases however knowledge is limited regarding bone health. Animal studies have shown garlic minimizes bone loss through increasing estrogen levels and reducing osteoclast bone resorption. Excessive bone loss can cause porous, brittle bones, commonly known as osteoporosis, which lead to high fracture risks. Utilizing natural alternatives to synthetic medicines can reduce physiological rejection while maintaining relief to ailments and diseases.

 

METHOD

One of the most well-known sulfur compounds extracted from garlic is allicin. The objective is to understand the effects of allicin release on the bone remodeling process. The hypothesis is allicin will show no cytotoxic effects to osteoblast cells and reduce osteoclast resorption. Allicin was extracted from pure garlic powder and loaded onto calcium phosphate scaffolds, mimicking bone tissue composition. Cellular and scaffold surface morphology were imaged post cell culture as well histological staining of an in vivo rat distal femur model.

 

RESULTS/IMPLICATIONS

Results show allicin has no cytotoxic effects on osteoblast morphology and a reduction of osteoclast resorption pit formation. Allicin also shows significantly enhanced collagen formation in vivo, indicating another avenue for improved bone healing. With these results, further knowledge is gained on the ability for garlic to improve bone health in bone tissue engineering applications.

 

Cooling Rates of Spatter Deposits

Cooling Rates of Spatter Deposits

Primary Author: Claire Puleio

Faculty Sponsor: Catherine Cooper

 

Primary College/Unit: Agricultural, Human and Natural Resource Sciences

Category: Agricultural and Natural Resource Sciences

Campus: Pullman

 

Abstract:

 

Principal Topic

Magmatic spatter deposits form during volcanic eruptions wherein molten lava is projected from the volcano. The molten lava is erupted in fragments (clasts) and is deposited in the area immediately surrounding the eruptive vent of the volcano. These clasts can pile upon each other and form cone-like structures. Magmatic spatter occurs when erupted lava is hot enough to deform and adhere to other erupted clasts (agglutinate). The deformation and agglutination of spatter clasts have important implications regarding how spatter can transition from a stable deposit to a lava flow. When spatter re-melts and flows it can cause sudden collapse of the cone-like structures and quickly damage infrastructure or cause bodily harm to those in the path of the flow.

 

Method

A two-dimensional thermal diffusion model has been created in this study to predict how long it takes for spatter clasts to cool sufficiently enough that they no longer pose the risk of re-melting and forming a lava flow. This model predicts how spatter clasts cool over time when subjected to conduction, convection, and radiation and is applied to scenarios in which multiple spatter clasts of the same temperature are placed on top of one another.

 

Results/Implications

The model described in this research provides an indication for when the spatter deposit will cool sufficiently enough to stabilize. This research increases the understanding of magmatic spatter as well as the likelihood for associated volcanic hazards such as sudden collapse of spatter deposits and the rapid formation of lava flows.

 

A better screening tool to help combat a common pest of wheat

A better screening tool to help combat a common pest of wheat

Primary Author: Samuel Prather

Faculty Sponsor: Michael Pumphrey

 

Primary College/Unit: Agricultural, Human and Natural Resource Sciences

Category: Agricultural and Natural Resource Sciences

Campus: Pullman

 

Abstract:

 

Principle topic:

Hessian Fly [Mayetiola destructor (Say)] is a major pest of wheat in Washington as well as the entire USA. In its larval stage, Hessian fly feeds off the stems of wheat plants causing severe yield loss. While there are pesticides and management practices to combat Hessian fly, because of Hessian fly’s unique life cycle most are not effective. The best way to combat Hessian fly is through use of genetically resistant wheat varieties with one of the 35 known Hessian fly resistance genes. The impediment for breeders developing Hessian fly resistance varieties is a fast-cost-effective way to screen for the resistance, as the current method takes a long time and is very expensive.

Method:

Using a genetics technique known as linkage mapping my project’s goal was to find the genetic location of one of the 35 known genes that has been shown to work in Washington. And then create genetic markers which are an assay to test for that gene.

Results:

After leaning the location of our Hessian fly resistance gene of interest I created 3 genetic markers and validated them on a large panel of varieties. The results show these markers to be highly (>98%) accurate at detecting the presence of the gene. The old method of testing for this gene used by our lab cost ~$150 per test and took about 2 months. This new method using the genetic marker assay takes less than a week and cost ~$1 per test.

 

Effects of Videocases on Teacher Learning and Classroom Practice: A Meta-Analysis

Effects of Videocases on Teacher Learning and Classroom Practice: A Meta-Analysis

Primary Author: Samuel Aina

Faculty Sponsor: Olusola Adesope

 

Primary College/Unit: College of Education

Category: Arts and Education Sciences

Campus: Pullman

 

Abstract:

Teachers need opportunity to learn and grow professionally to be effective and help students learn. Studies have shown that videocase analysis, the process of having teachers record, watch and analyze their own teaching, is a powerful approach to developing teacher quality This approach fosters teachers’ critical thinking, self-reflection, professional vision and practice, with the goal of improving learning opportunities for students. Many studies have reported positive research findings that support videocase analysis as an effective teacher development tool. However little is known about how contextual factors affect the effectiveness of videocases. Do teachers learn better when they watch their own video or a professionally shot video of other teachers? Should they watch their video alone or with other colleagues? How do these and other contextual factors help teachers learn better from videocases? This meta-analysis examined the aggregate effects of videocases on teachers’ learning and practice. Results from 27 studies included in the meta-analysis showed that videocases are more beneficial for teacher learning and practice than other comparison conditions (g = 0.65, p <.001). Findings show how the mean effect sizes were moderated by contextual variables such as video source, video recording type, participant characteristics, study setting and methodological features of the studies. The study concludes with the implications of the meta-analysis for scientific inquiry, classroom practice and education policy.

 

Safe by comparison: Unintended Consequences of the Effects of Comparison Between Alternative Tobacco Products.

Safe by comparison: Unintended Consequences of the Effects of Comparison Between Alternative Tobacco Products.

Primary Author: Kamal Ahmmad

Faculty Sponsor: Elizabeth Howlett

 

Primary College/Unit: Carson College of Business

Category: Business, Communication, and Politial Sciences

Campus: Pullman

 

Abstract:

Principle topic: Graphic Health Warnings (GHWs) on cigarette packages are used to discourage smoking. However, the use of GHWs on cigarette packages may have unintended negative consequences. We examined how GHWs on cigarette packages can bias consumers’ evaluation of e-cigarettes. Negative emotions such as fear, guilt, and disgust generated by warnings and disclosures on cigarette packages lead to changes in cognitions, judgments, and behaviors (Andrews et al 2014, Netemeyer et al 2016). Similarity and preference judgment literature also posit that consumers’ engage in comparison processes when they asses product similarity (Simonson & Tversky 1992. Hagius & Mason 1993). Since cigarettes and e-cigarettes are two similar product and most smokers switch to e-cigarettes as a means to quit smoking, counter-marketing of cigarettes with GHWs would influence the evaluation of e-cigarettes.

Method: We conducted two online studies and one lab study to examine the mechanism through which GHWs influence e-cigarette-related consumer responses. In addition to testing behavioral intention related to e-cigarettes, we also tested consumers’ information seeking behavior in response to GHWs on cigarette pack.

Results and Implications: Results from three studies show that GHWs on cigarette packages increase cigarette related fear and decrease e-cigarette related fear. The elicited fear influences attitudes and health hazard beliefs related to e-cigarettes. We also find that GHWs on cigarettes increase the information seeking behavior related to e-cigarettes. The results have significant policy implications which show that counter-marketing efforts of one harmful products have unintended negative consequences by increasing the preference for another potentially harmful product.

 

Enhancing Mass Transfer of Nutraceuticals to Inflamed Cartilage Cells through Perfusion

Enhancing Mass Transfer of Nutraceuticals to Inflamed Cartilage Cells through Perfusion

Primary Author: Haneen Abusharkh

Faculty Sponsor: Bernard Van Wie

 

Primary College/Unit: Voiland College of Engineering and Architecture

Category: Engineering and Environmental Science

Campus: Pullman

 

Abstract:

Articular cartilage is a connective tissue that lacks blood vessels or sensory neurons. The lack of vascularity presents cartilage with diffusion-limited nutrient and oxygen supply and minimal intrinsic ability to regenerate after injury, leading to Osteoarthritis (OA). The aneural nature of cartilage makes injury difficult to diagnose due to lack of pain and therefore OA intervention has a tendency to be delayed. OA is the most common joint disease in the U.S. and was traditionally defined solely as the degradation of cartilage and was not considered an inflammatory disease. However, several recent studies have proven the presence of inflammatory markers, including interleukins, in the serum of OA joints. These findings have transformed how researches define and develop treatments for OA.

Nutraceuticals are food components that have medicinal benefits in addition to their nutritional value. They reduce inflammation by blocking the expression of interleukin-1 and scavenge reactive oxygen species (ROS) and free radicals by their anti-oxidative characteristics.

In this study, inflammation was induced in bovine cartilage cells by the addition of interleukin-1β. Then, cells were cultured in two groups, a static micromass, and a perfusion bioreactor group. Both groups were supplied with a nutraceutical containing growth medium. We hypothesized that perfusion enhances the mass transfer of nutraceuticals to the grown cartilage tissue and reverses the inflammatory symptoms. Our results suggest that inflammation was reduced in the bioreactor samples, reflected by higher production of proteins indicative of healthy cartilage, collagen, and glycosaminoglycan, by more than 16-fold in comparison to static micromass cultures.